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SOD activity was significantly decreased by approximately 19 % in the LPS-treated cells compared to the control (Figure 1B)
Chronic Oxidative Stress (Depletes Glutathione Faster Than Its Made) Anything that increases free radical production depletes glutathione: Chronic inflammation (autoimmune diseases, obesity, metabolic syndrome) Environmental toxins (air pollution, pesticides, heavy metals) Alcohol consumption (glutathione is rapidly depleted in liver detoxification) Poor diet (high in processed foods, seed oils, sugar) Intense exercise (without adequate recovery) When oxidative stress exceeds your bodys capacity to recycle GSSG back to GSH, levels drop [4]
Next, the lysate was centrifuged at 12,000 g for 10 min at 4C
Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center
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