Chondrocyte dedifferentiation was majorly characterized as the reduced expression of COL2 and ACAN and enhanced expression of type I collagen and versican ( COL2 expression, but not ACAN ( In addition to dedifferentiation, another consequence of in vitro expansion is replicative senescence, which also significantly influences chondrocyte function
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Proteoliposome validation To ensure efficient protein reconstitution, 100 l frozen proteoliposomes were thawed and a 10 l aliquot was collected for Western blot analysis
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