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The cell pellet was resuspended and lysed in lysis buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 1% Triton-X100 (v/v), 2 mM MgCl 2 , 0.5 mM TCEP) with protease inhibitors (1 mM PMSF, 1 M pepstatin, 4 M leupeptin, 0.3 M aprotinin) for 30 min at 4 C

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Patients consuming sulfur-rich diets are particularly susceptible to fermentation-related symptoms because transit delay amplifies bacterial exposure time" [4]

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In a group of adult PWS subjects, GH treatment for 2 years did not effectively improve low bone mass (86)