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Following cleavage with Precision Protease (Beyotime) to remove the GST tag, the eluted proteins underwent further purification by size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) using an elution buffer of 50 mM HEPES (pH 7.4) and 150 mM NaCl

Kostenuik PJ, Harris J, Halloran BP, Turner RT, Morey-Holton ER, Bikle DD

marneffei , which were designated as TmGst1-TmGst20

Han, Chong

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