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Copper (I) oxide causes dose-dependent decrease in the viability of airway epithelial cells by impairing the activities of catalase and glutathione reductase , generating reactive oxygen species , and increasing the levels of 8-isoprostane and the ratio of oxidized glutathione

Since the cellular synthesis of glutathione is ATP-dependent, decreased ETC activity also undermines glutathione synthesis, further exacerbating oxidative stress in a vicious cycle [8]

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A single freeze-thaw cycle can reduce GHK-Cu activity by 2030%