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Frequent mutation of the PI3K pathway in head and neck cancer defines predictive biomarkers

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Following the final centrifugation at 160 g for 3 minutes, the cell pellet was resuspended, counted, and replated at a concentration of 1 x 10 5 cells/cm 2 in two of the four tested conditions: (1) N2B27 medium (see above) containing 10 g/ml BDNF (Qkine) (NB) and (2) co-cultures of hPSC-derived neurons with primary mouse astrocytes in N2B27 medium (NBA)

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