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Restoring vitamin B12 to healthy levels can treat anemia

Nature 2014;510:412-6

When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature

If you've used large water volumes, light blue color may be normal

There is some evidence that a sufficient daily dietary intake of cysteine may reduce the requirement for methionine (Di Buono et al, 2001)

Patent data (HPLC-verified): This is a genuine practical advantage for oral dosing the arginate form survives stomach acid roughly 1,000x better than acetate at pH 3.0