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doi:10.1016/S0140-6736(08)60073-2

however, Cu/Zn-SOD knockout mice were found to be expected and show changes only after traumatic injury [82]

Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Do not exceed the stated recommended daily dose

Samples were then washed with PBS as described previously and incubated with goat anti-rabbit secondary antibody (1:500 dilution, Beyotime) at room temperature for 1 h in darkness