J Biol Chem 279: 77857791
Cells were incubated for 1 h in darkness with an anti-rabbit AF555 secondary antibody (1:300, Thermo Fischer Scientific, A31572) and an anti-HA AF488 conjugate (1:1000) diluted in blocking buffer with the latter intended to label HA-tagged S associated with intracellular membranes (as well as available external HA-S epitopes)
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Eser TM, Baranov O, Huth M, Ahmed M, Dek F, Held K, et al

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Every client will require a specific dose of Semaglutide, but it is typically administered weekly